The ability to form peptide bonds to bind amino acids together is more than 100 years old, though the first peptides to be synthesized, including oxytocin and insulin, did not occur for another 50-60 years, demonstrating the difficult task of chemically synthesizing amino acid chains. Over the last 50 years, advances in the chemistry and methods of protein synthesis have developed to the point where peptide synthesis is a common approach in even high-throughput biological research and product and drug development. The benefit of peptide synthesis techniques today is that in addition to being able to create peptides present in biological specimens, ingenuity and innovation can be tapped to generate new peptides to maximize a desired biological response or other result. This page highlights the important aspects of peptide synthesis, the most popular synthesis and purification methods, as well as the strengths and shortcomings of the strategies involved.
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